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| Registros recuperados: 40 | |
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| Molinari, Nicolas; Abou-badra, Mathieu; Marin, Gregory; Ky, Chin-long; Amador, Noemi; Gamez, Anne Sophie; Vachier, Isabelle; Bourdin, Arnaud. |
| Background: COPD is largely under-diagnosed and once diagnosed usually at a late stage. Early diagnosis is thoroughly recommended but most attempts failed as the disease is marginally known and screening marginally accepted. It is a rare cause of concern in primary care and spirometry is not very common. Exhaled carbon monoxide (eCO) is a 5-seconds easy-to-use device dedicated to monitor cigarette smoke consumption. We aimed to assess whether systematic eCO measurement in primary care is a useful tool to improve acceptance for early COPD diagnosis. Methods: This was a two-center randomized controlled trial enrolling 410 patients between March and May, 2013. Whatever was the reason of attendance to the clinic, all adults were proposed to measure eCO during... |
| Tipo: Text |
Palavras-chave: COPD; Screening; Exhaled CO; Smoking; Behaviour. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38142/36280.pdf |
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| Ky, Chin-long; Le Pabic, Lore; Koua, Manaarii Sham; Molinari, Nicolas; Nakasai, Seiji; Devaux, Dominique. |
| The black-lipped pearl oyster, Pinctada margaritifera is the most important farmed mollusc species in French Polynesia. Donor oyster selection among wild P. margaritifera individuals, chosen according to their inner shell colour, makes it possible to obtain the broadest range of cultured pearl colours of any species. This study demonstrates the relative influence of using black [B] or red [R] outer shell phenotypes, combined with green [G] or yellow [Y] inner shell phenotypes, on pearl darkness level, colour categories and lustre. A large scale grafting experiment was designed and carried out over five grow-out locations, covering three archipelagos: Tuamotus, Society and Gambier. Results revealed that the [B + G] phenotypes may be used as donors to... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Shell colour phenotype; Pearl colour; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00285/39637/38129.pdf |
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| Le Moullac, Gilles; Soyez, Claude; Ky, Chin-long. |
| The pearl oyster is one of the rare animal models that support two distinct genomes, through the surgical graft process operated for culture pearl production. This grafted organism is assimilated to a chimera whose physiological functioning remains poorly known. The question of the energy expenditure comparison between chimera and non-chimera animals arises. To answer this question, grafted and non-grafted pearl oysters were evaluated for their energetic needs by the indirect calorimetry method. This method made it possible to measure the energy expenditure based on the respiration rate (RR) measurement, reflecting the basal metabolism. The results showed that the RR values for grafted and non-grafted pearl oysters were not significantly different... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00437/54861/56350.pdf |
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| Ky, Chin-long; Blay, Carole; Aiho, Vaite; Cabral, Philippe; Le Moullac, Gilles; Lo, Cedrik. |
| In French Polynesia, the aquaculture of P. margaritifera is carried out in numerous grow-out sites, located over three archipelagos (Gambier, Society and Tuamotu). To evaluate the impact of macro-geographical effects of these growing sites on pearl quality traits, five hatcheries produced families were used as homogeneous donor oysters in an experimental graft. The molluscs were then reared in two commercial locations: Tahaa island (Society) and Rangiroa atoll (Tuamotu). At harvest, eight pearl quality traits were recorded and compared: surface defects, lustre, grade, circles, shape categories, darkness level, body and secondary colour and visual colour categories. Overall inter-site comparison revealed that: 1) all traits were affected by grow-out... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl quality; Grow-out location; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00276/38687/37186.pdf |
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| Dubé, Caroline; Ky, Chin-long; Planes, Serge. |
| Elucidating the role of prokaryotic symbionts in mediating host physiology has emerged as an important area of research. Since oysters are the world’s most heavily cultivated bivalve molluscs, numerous studies have applied molecular techniques to understand the taxonomic and functional diversity of their associated bacteria. Here, we expand on this research by assessing the composition and putative functional profiles of prokaryotic communities from different organs/compartments of the black-lipped pearl oyster Pinctada margaritifera, a commercially important shellfish valued for cultured pearl production in the Pacific region. Seven tissues, in addition to mucous secretions, were targeted from P. margaritifera individuals: the gill, gonad, byssus gland,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Microbiome; Tissue-specific bacterial communities; 16S rRNA gene sequencing; Functional profiling prediction. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61670/65589.pdf |
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| Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Duboisset, Julien; Dicko, Hamadou; Durand, Patrick; Quintric, Laure; Planes, Serge; Vidal-dupiol, Jeremie. |
| The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Color; Pigment; Transcriptomics; Raman spectroscopy. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00685/79671/82446.pdf |
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| Ky, Chin-long; Lo, Cedrik; Planes, Serge. |
| The pearl oyster Pinctada margaritifera has the specific ability to produce pearls with the widest range of colours among all pearl oyster species. This pearl colour diversity originates from the mantle biomineralising tissue (graft) of the donor oyster, which is originally responsible for the variety of colours of the inner shell surface. This study aimed to: 1) assess the geographic distribution and establish a first stocklist of the colourful oyster phenotypes used as donors in French Polynesia, and 2) investigate the phenotypic relation between inner shell colouration and the corresponding colour of harvested pearls. With the support of a pearl farmers' network, we investigated the different donor phenotype frequencies among five collection sites (Ahe,... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00354/46474/46249.pdf |
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| Blay, Carole; Planes, Serge; Ky, Chin-long. |
| Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters. Several phenotypic measurements were made: 1) donor family growth performance from shell size records, 2) pearl size and corresponding quality traits, and 3) expression of some genes related to... |
| Tipo: Text |
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| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00444/55593/57220.pdf |
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| Ky, Chin-long; Quillien, Virgile; Broustal, Floriane; Soyez, Claude; Devaux, Dominique. |
| The bivalve Pinctada margaritifera exhibits three main transplant phenotypes derived from the donor (from which a mantle graft tissue, the saibo, is excised), the recipient (into which the saibo is implanted with a nucleus, leading to the formation of a pearl sac "chimera") and the cultured pearls themselves. This first phenome study on the species derived from a large experimental graft. Transplant phenotype was assessed at three scales: 1) macro, pearl size, colour, grade, 2) micro, pearl surface microstructure, and 3) molecular, biomineralisation gene expression level in saibo and pearl sac tissues. From donor to pearl, the phenome revealed fine variations of quality traits dependent on the position on the mantle where the saibo was cut, whose... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53419/54306.pdf |
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| Ky, Chin-long; Leclerc, Nicolas; Broustal, Floriane; Nakasai, Seiji; Devaux, Dominique. |
| Aquaculture of nacreous gems, such as cultured pearls, mabé or keshi, is done mostly using different mollusc species grown in countries of the Indo-Pacific region. To date, no single species has been exploited for the simultaneous generation of more than one of these bioproducts, but all require animals with colourful shells. Historically, Pinctada species have mainly been used for nucleated pearl production, selecting the rarer colourful individuals to be used as graft donors. By contrast, colourful Pteria species have mostly been used for mabé production, as the grafting operation for pearl production is associated with low yield. In this study, we report the potential for cumulating cultured pearl and mabé (MP), or keshi and mabé (MK) production, using... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Mabe; Keshi; Pearls. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00482/59398/62168.pdf |
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| Ky, Chin-long; Okura, Retsu; Nakasai, Seiji; Devaux, Dominique. |
| In French Polynesia, the aquaculture of Pinctada margaritifera (Linnaeus, 1758) covers a large maritime exploitation area, spread over nearly 20 degrees latitude and longitude, with numerous pearl farms located in three archipelagos (Gambier, Society, and Tuamotu). As these archipelagos have specific seasonal temperature patterns each year, pearl oysters are subject to disparate and contrasting environmental regimes. This study aimed to examine the specificity of commercial pearl quality traits (n ¼ 2,236 samples) at the archipelago scale, in such a way as to provide preliminary data to design the most appropriate strategy for the distribution of hatchery-produced phenotypes. A large and standardized grafting experiment using the same donor phenotype was... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Large-scale geographic effects; Pearl color; Pearl grade; Pearl shape; Pearl size. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00357/46798/46665.pdf |
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| Ky, Chin-long; Blay, Carole; Broustal, Floriane; Sham Koua, Manaarii; Planes, Serge. |
| Molluscs display a vast range of shell colours both between and within species. However, only a few species show colour variation in their soft tissues. In French Polynesia, the pearl oyster Pinctada margaritifera has three tissue morphotypes: the black wild-type and two rare mutations: white albino and orange mantle. Phenotypic transmission is known to occur from these phenotypes when they are used as graft donors for pearl production, leading to multicoloured and white pearls from black and albino mantle grafts, respectively. The present study furthers this knowledge by examining the phenotypic association between the orange mantle tissue morphotype and hard tissues: shells and cultured pearls. Based on a large experimental graft, shell colour... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00486/59760/62878.pdf |
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| Ky, Chin-long; Le Moullac, Gilles. |
| Size is the most important and valuable quality trait of cultured pearls produced by the black-lipped pearl oyster, Pinctada margaritifera. In French Polynesia, several breeding programmes have been started that aim to improve this size trait, which is highly related to shell growth rate in both recipient and donor oysters. Shell growth rate dictates the time of grafting, size of implanted nuclei and bio-mineralisation potential of the mantle and pearl sac. We assessed shell growth rate through routine digital shell biometric analysis on 22 hatchery families produced between 2005 and 2008. These included full-sib families and half-sib families derived from polyandry (one dam crossed with two or more sires). Results showed that: 1) a significant family... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Hatchery-produced families; Growth performance; Shell diameter; Cultured pearl size. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49810/50384.pdf |
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| Reisser, Celine; Lo, Cédrik; Schikorski, David; Sham Koua, Manaarii; Planes, Serge; Ky, Chin-long. |
| The French Polynesian islands are internationally known for their black pearls, produced by culture of the black lipped pearl oyster Pinctada margaritifera. The ongoing development of hatcheries for P. margaritifera in French Polynesia poses new challenges for the industry, particularly regarding the maintenance of genetic diversity in the hatchery stocks. This emphasizes the necessity to characterize the genetic diversity and differentiation within natural and exploited populations, to carefully select putative parental populations. The present study aimed at validating the phylogenetic status and investigating genetic attributes of populations from the only two non-exploited archipelagos of French Polynesia, the Marquesas archipelago, and the Australes... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00509/62059/66234.pdf |
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| Joubert, Caroline; Linard, Clementine; Le Moullac, Gilles; Soyez, Claude; Saulnier, Denis; Teaniniuraitemoana, Vaihiti; Ky, Chin-long; Gueguen, Yannick. |
| In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00210/32095/30535.pdf |
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| Ky, Chin-long; Broustal, Floriane; Potin, Dan; Lo, Cedrik. |
| In French Polynesia, the P. margaritifera pearl aquaculture industry is spread over a vast area, as large as Europe. All the oysters for this the highly economically important activity are supplied from just a few collection lagoons, but they are grown in numerous sites across three archipelagos (Gambier, Society and Tuamotu). Many oyster transfers thus indirectly bring about grafting combinations mixing different geographic origins and production sites. This study aims to examine the impact of such graft combinations on cultured pearl quality traits. For this, six homogeneous and standardised experimental graft combinations (N = 6197) were conducted at commercial scale in the two growing locations the most frequently used in French Polynesia: Arutua atoll... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Graft combinations; Pearl quality traits; Environment. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00477/58888/61444.pdf |
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| Auffret, Pauline; Le Luyer, Jeremy; Sham Koua, Manaarii; Quillien, Virgile; Ky, Chin-long. |
| Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Albinism; Pigmentation; RNA-seq; Notch signaling pathway; Tyrosinase; Biomineralization. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00652/76420/77477.pdf |
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| Ky, Chin-long; Nakasai, Seiji; Parrad, Sophie; Broustal, Floriane; Devaux, Dominique; Louis, Patricia. |
| Cultured pearl production from Pinctada margaritifera uses the biomineralization capacities of the mantle graft, the saibo, which is usually obtained from only the middle mantle section of the donor oyster. To evaluate the potential for using other parts of the mantle, this study explores and describes the cultured pearl quality traits, pearl size, shape, surface defects and colour parameters obtained with saibo from the entire length of the mantle, comprising the four following sections: 1) posterior, 2) connection with the gills, 3) middle, (the section usually used commercially), and 4) anterior. Rates of nucleus retention and oyster mortality were also recorded and compared between sections. For this, two experimental grafts were designed and conducted... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Pearl quality traits; Saibo position; Mantle tissue; Phenotype. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00439/55030/56461.pdf |
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| Le Luyer, Jeremy; Auffret, Pauline; Quillien, Virgile; Leclerc, Nicolas; Reisser, Celine; Vidal-dupiol, Jeremie; Ky, Chin-long. |
| Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the Pinctada genus, through the biomineralization properties of pearl sac tissue. Improvement of P. margaritifera pearl quality is one of the biggest challenges that Polynesian research has faced to date. To achieve this goal, a better understanding of the complex mechanisms related to nacre and pearl formation is essential and can now be approached through the use of massive parallel sequencing technologies. The aim of this study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that had producing pearls with high and low quality. For this purpose, a comprehensive reference transcriptome of P. margaritifera was built based on... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Biomineralization; Pearl quality; Pearl sac; RNA-seq. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00475/58695/61207.pdf |
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| Registros recuperados: 40 | |
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